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Image Search Results
Journal: Cancers
Article Title: Preclinical Evaluation of the Novel Small-Molecule MSI-N1014 for Treating Drug-Resistant Colon Cancer via the LGR5/β-catenin/miR-142-3p Network and Reducing Cancer-Associated Fibroblast Transformation
doi: 10.3390/cancers12061590
Figure Lengend Snippet: Cancer-associated fibroblasts (CAFs) increased the oncogenic properties of colon cancer cells. ( A ) Insert illustrates the co-culture system of DLD1 and HCT116 colorectal cancer (CRC) cells with Normal fibroblasts (NF) and CAFs. Cell viability assay showed increased 5-fluorouracil (5-FU) resistance in CAF-educated DLD1 and HCT116 cells, compared to their naïve counterparts. Flow cytometric analysis of DLD1 and HCT116 cells co-cultured with CAFs. Increased CD44 + cell population ( B ) and side population ( C ) in both DLD1 and HCT116 cells, 48 h post CAF culture. Enhanced colony-forming ( D ), migratory ( E ), and tumor sphere-generating ( F ) abilities in both CRC cell lines post CAF co-culture. ** p < 0.01, *** p < 0.001.
Article Snippet: The DLD1 and HCT116 human colon cancer cell lines and
Techniques: Co-Culture Assay, Viability Assay, Cell Culture